Optimization of hyaluronan-based eye drop formulations

Hyaluronan (HA) is frequently incorpor ated in eye drops to extend the pre-corneal residence time, due to its viscosifying and mucoa dhesive properties. Hydrodynamic and rheological evaluations of commercial products are first ac complished revealing molecular weights varying from about 360 to about 1200 kDa and viscosity values in the range 3.7-24.2 mPa s. The latter suggest that most products could be optimized towards resistance to drainage from the ocular surface. Then, a study aiming to maximize the viscosity and mucoadhesiveness of HA-based preparations is performed. The effect of polymer ch ain length and concentration is investigated. For the whole range of molecular weights encounte red in commercial products, the concentration maximizing performance is identified. Such concentration varies from 0.3 (wt%) for a 1100kDa HA up to 1.0 (wt%) for a 250kDa HA, which is 3-fold higher than the highest concentration on the market. The viscosity and mucoadhesion profiles of optimized formulations are superior than commercial products, especial ly under conditions simulating in vivo blinking. Thus longer retention on the corneal epithelium can be predicted. An enhanced capacity to protect corneal porcine epithelial cells from dehydration is also demonstrated in vitro . Overall, the results predict formulations with improved efficacy