We investigated the effect of PS (1) on nitrite and reactive oxygen species (ROS) production in J774 macrophages stimulated with bacterial lipopolysaccharide (LPS) for 24 h. PS (1) inhibited in a concentration-dependent manner nitrite and ROS production as well as inducible nitric oxide synthase (iNOS) protein expression induced by LPS. Incubation of cells with PS (1) determined a significant decrease of nuclear factor-kappa B (NF-kappa B)/DNA binding activity which was correlated with a marked reduction of iNOS mRNA levels. These results show that PS (1) inhibits NF-kappa B activation and iNOS gene expression by preventing the reactive species production and suggest a role for this compound in controlling oxidative stress and/or inflammation.
A Polysaccharide from tomato (Lycopersicon esculentum) peels affects NF-kappaB activation in LPS-stimulated J774 macrophages
Simeon, VittorioInvestigation
;
2007
Abstract
We investigated the effect of PS (1) on nitrite and reactive oxygen species (ROS) production in J774 macrophages stimulated with bacterial lipopolysaccharide (LPS) for 24 h. PS (1) inhibited in a concentration-dependent manner nitrite and ROS production as well as inducible nitric oxide synthase (iNOS) protein expression induced by LPS. Incubation of cells with PS (1) determined a significant decrease of nuclear factor-kappa B (NF-kappa B)/DNA binding activity which was correlated with a marked reduction of iNOS mRNA levels. These results show that PS (1) inhibits NF-kappa B activation and iNOS gene expression by preventing the reactive species production and suggest a role for this compound in controlling oxidative stress and/or inflammation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
