Over 300 non redundant SSR markers were identified by bioinformatic tools. Simple sequence repeats were identified in genomic and EST sequences of Phytophthora infestans, the causal agent of ‘potato late blight’. Primers were developed and tested on a reference set of P. infestans isolates. Finally, eight most polymorphic and robust scoring SSR markers were assembled into two multiplex sets using fluorescent primers. These microsatellite markers were used for monitoring population diversity of P. infestans isolates in several parts of the world. In a survey of Dutch late blight populations from 2004 to 2007, nearly 500 fungal isolates were collected from commercial potato crops. Analysis of these eight polymorphic SSRs among Dutch isolates revealed clonal lineages. Multilocus analysis confirmed the clonality of this pathogen in the Netherlands. The genetic structure of Dutch isolates was of intermediate complexity, which included multiple, closely related genotypes. The analysis of population migration in diverse continents, emphasized the role of commercial plant trade in the movement of this pathogen. Objective of this work was to generate SSR markers that would allow efficient monitoring of worldwide populations of P. infestans.
Microsatellite markers from genome sequences for Phytophthora infestans population analysis
Testa A.;
2009
Abstract
Over 300 non redundant SSR markers were identified by bioinformatic tools. Simple sequence repeats were identified in genomic and EST sequences of Phytophthora infestans, the causal agent of ‘potato late blight’. Primers were developed and tested on a reference set of P. infestans isolates. Finally, eight most polymorphic and robust scoring SSR markers were assembled into two multiplex sets using fluorescent primers. These microsatellite markers were used for monitoring population diversity of P. infestans isolates in several parts of the world. In a survey of Dutch late blight populations from 2004 to 2007, nearly 500 fungal isolates were collected from commercial potato crops. Analysis of these eight polymorphic SSRs among Dutch isolates revealed clonal lineages. Multilocus analysis confirmed the clonality of this pathogen in the Netherlands. The genetic structure of Dutch isolates was of intermediate complexity, which included multiple, closely related genotypes. The analysis of population migration in diverse continents, emphasized the role of commercial plant trade in the movement of this pathogen. Objective of this work was to generate SSR markers that would allow efficient monitoring of worldwide populations of P. infestans.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
