ZBTB2 protein is a new partner of the Nucleosome Remodeling andDeacetylase (NuRD) complex

ZBTB2 is a protein belonging to the BTB/POZ zinc-finger family whose members typically contain a BTB/POZ do-main at the N-terminus and several zinc-finger domains at the C-terminus. Studies have been carried out to dis-close the role of ZBTB2 in cell proliferation, in human cancers and in regulating DNA methylation. Moreover,ZBTB2 has been also described as an ARF, p53 and p21 gene repressor as well as an activator of genes modulatingpluripotency. In this scenario, ZBTB2 seems to play many functions likely associated with other proteins. Here wereport a picture of the ZBTB2 protein partners in U87MG cell line, identified by high-resolution mass spectrom-etry (MS) that highlights the interplay between ZBTB2 and chromatin remodeling multiprotein complexes.In particular, our analysis reveals the presence, as ZBTB2 candidate interactors, of SMARCA5 and BAZ1B compo-nents of the chromatin remodeling complex WICH and PBRM1, a subunit of the SWI/SNF complex. Intriguingly,we identified all the subunits of the NuRD complex among the ZBTB2 interactors. By co-immunoprecipitation ex-periments and ChIP-seq analysis we definitely identify ZBTB2 as a new partner of the NuRD complex.