Agrobacterium tumefaciens F/1 produces two different O-chains, both are constituted of rhamnose and glucosamine: the less abundant has a linear disaccharidic repeating unit 3)-α-l-Rhap-(1 → 3)-β-d-GlcpNAc- (1→ and the second one 4)-α-l-Rhap-(1 → 3)-β-d-GlcpNAc- (1→. The two intact antigenic moieties were studied in mixture by 2D NMR. Additional supporting data were obtained by periodate degradation, the major component was cleaved selectively, leading to a glucosamine glycoside, whereas the minor one was recovered unaffected. © 2004 Elsevier Ltd. All rights reserved.
Elucidation of two O-chain structures from the lipopolysaccharide fraction of Agrobacterium tumefaciens F/1
Piscopo V.;
2004
Abstract
Agrobacterium tumefaciens F/1 produces two different O-chains, both are constituted of rhamnose and glucosamine: the less abundant has a linear disaccharidic repeating unit 3)-α-l-Rhap-(1 → 3)-β-d-GlcpNAc- (1→ and the second one 4)-α-l-Rhap-(1 → 3)-β-d-GlcpNAc- (1→. The two intact antigenic moieties were studied in mixture by 2D NMR. Additional supporting data were obtained by periodate degradation, the major component was cleaved selectively, leading to a glucosamine glycoside, whereas the minor one was recovered unaffected. © 2004 Elsevier Ltd. All rights reserved.File in questo prodotto:
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