The RADiation sensitive52 (RAD52) protein catalyzes the pairing between two homologous DNA sequences’ double-strand break repair andmeiotic recombination, mediating RAD51 loading onto single-stranded DNA ends, and initiating homologous recombination and catalyzing DNA annealing. This article reports the characterization of RAD52 homologs in the thermo-acidophilic Cyanidiophyceae whose genomes have undergone extensive sequencing. Database mining, phylogenetic inference, prediction of protein structure and evaluation of gene expression were performed in order to determine the functionality of the RAD52 protein in Cyanidiophyceae. Its current function in Cyanidiophytina could be related to stress damage response for thriving in hot and acidic environments as well as to the genetic variability of these algae, in which, conversely to extant Rhodophyta, sexual mating was never observed.
A spotlight on Rad52 in cyanidiophytina (Rhodophyta): A relic in algal heritage
Iovinella M;Petriccione M;Ciniglia C
Writing – Review & Editing
2019
Abstract
The RADiation sensitive52 (RAD52) protein catalyzes the pairing between two homologous DNA sequences’ double-strand break repair andmeiotic recombination, mediating RAD51 loading onto single-stranded DNA ends, and initiating homologous recombination and catalyzing DNA annealing. This article reports the characterization of RAD52 homologs in the thermo-acidophilic Cyanidiophyceae whose genomes have undergone extensive sequencing. Database mining, phylogenetic inference, prediction of protein structure and evaluation of gene expression were performed in order to determine the functionality of the RAD52 protein in Cyanidiophyceae. Its current function in Cyanidiophytina could be related to stress damage response for thriving in hot and acidic environments as well as to the genetic variability of these algae, in which, conversely to extant Rhodophyta, sexual mating was never observed.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
