Association between 14bp insertion/deletion HLA-G functional polymorphism and insulin-resistance in a cohort of Italian children with obesity.

BACKGROUND: The non-classical HLA-class I molecule-g (HLA-G) gene shows a deletion/insertion (del/ins) polymorphism of a 14 base pairs sequence (14bp) in the exon 8 at the 3'untranslated region. The presence of the 14bp insertion allele has been associated to lower soluble HLA-G protein production, a protein with anti-inflammatory activities. So far, no studies have investigated the relationship between HLA-G 14bp del/ins polymorphism and metabolic features of obese children and adolescents. OBJECTIVE: To assess if the HLA-G ins/del polymorphism, and in particular the HLA-G ins/ins genotype determining lower sHLA-G production, is associated to insulin-resistance (evaluated by homeostasis model assessment [HOMA]) in a population of obese children. SUBJECTS: We enrolled 574 obese children and adolescents. METHODS: Anthropometric and laboratory data were collected. The white blood cell (WBC) count was evaluated as surrogate marker of inflammation. C-reactive protein (CRP) was available in 48 patients. HOMA was calculated. Patients were genotyped for the HLA-G del/ins polymorphism. RESULTS: Subjects carrying the HLA-G ins/ins genotype, presented with higher HOMA, WBC and CRP values, compared to del/ins and del/del genotypes (p≤0.0009, ≤0.02 and ≤0.0001, respectively). Comparison of the regression line slopes, performed for HOMA and WBC on the basis of HLA-G genotypes, showed that subjects carrying the HLA-G ins/ins genotype presented with a stronger correlation between HOMA and WBC, compared to the other genotypes (Model r2 3.13%, p≤0.006). CONCLUSIONS: We showed a strong association between HLA-G 14bp ins/ins genotype and HOMA in obese children and adolescents. This association could be hypothetically modulated by subclinical inflammation. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.