G-quadruplex structures formed by oligodeoxyribonucleotides TGGUNH2GGT (AM, UNH2 = 5-amino-2′-deoxyuridine), TGGUBrGGT (BR, UBr = 5-bromo-2′-deoxyuridine) and TGGTGGT (TH) have been investigated through circular dichroism, nuclear magnetic resonance, gel electrophoresis and molecular modeling techniques. Collected data indicate that all 7-mer oligonucleotides form tetramolecular parallel G-quadruplex structures with all residues adopting anti glycosidic bonds. In the case of AM, data suggest the occurrence of a novel UNH2-tetrad characterized by eight hydrogen bonds that stabilizes the G-quadruplex structure more efficiently than UBr- and T-tetrads.

A novel pyrimidine tetrad contributing to stabilize tetramolecular G-quadruplex structures

FILOSA, Rosanna;
2016

Abstract

G-quadruplex structures formed by oligodeoxyribonucleotides TGGUNH2GGT (AM, UNH2 = 5-amino-2′-deoxyuridine), TGGUBrGGT (BR, UBr = 5-bromo-2′-deoxyuridine) and TGGTGGT (TH) have been investigated through circular dichroism, nuclear magnetic resonance, gel electrophoresis and molecular modeling techniques. Collected data indicate that all 7-mer oligonucleotides form tetramolecular parallel G-quadruplex structures with all residues adopting anti glycosidic bonds. In the case of AM, data suggest the occurrence of a novel UNH2-tetrad characterized by eight hydrogen bonds that stabilizes the G-quadruplex structure more efficiently than UBr- and T-tetrads.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11591/362456
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