Functional Binding Surface of a beta-Hairpin VEGF Receptor Targeting Peptide Determined by NMR Spectroscopy in Living Cells

In this study, the functional interaction of HPLW peptide with VEGFR2 (Vascular Endothelial Growth Factor Receptor 2) was determined by using fast 15N-edited NMR spectroscopic experiments. To this aim, 15N uniformly labeled HPLW has been added to Porcine Aortic Endothelial Cells. The acquisition of isotope-edited NMR spectroscopic experiments, including 15N relaxation measurements, allowed a precise characterization of the in-cell HPLW epitope recognized by VEGFR2.