""We investigate the interactions between proteins and DNA in solutions of living cells, triggered by UV, 200-fs laser. pulses. DNA-protein crosslinking is obtained with two different. techniques: conventional chemical or laser-induced. Our results. evidence a nonlinear response of the cells to the laser irradiation. in the investigated intensity range. The laser induced crosslink. efficiency can reach a value approximately twice higher than. that obtained with ordinary chemical methods. The experimental. results are very well reproduced by a simple phenomenological. model based on the interplay between two-photon absorption by DNA bases and cell damage induced by high intensity laser pulse.. ""
Nonlinear protein – nucleic acid crosslinking induced by femtosecond UV laser pulses in living cells
NEBBIOSO, Angela;Benedetti R;Carafa V;Conte M;ALTUCCI, Lucia;
2012
Abstract
""We investigate the interactions between proteins and DNA in solutions of living cells, triggered by UV, 200-fs laser. pulses. DNA-protein crosslinking is obtained with two different. techniques: conventional chemical or laser-induced. Our results. evidence a nonlinear response of the cells to the laser irradiation. in the investigated intensity range. The laser induced crosslink. efficiency can reach a value approximately twice higher than. that obtained with ordinary chemical methods. The experimental. results are very well reproduced by a simple phenomenological. model based on the interplay between two-photon absorption by DNA bases and cell damage induced by high intensity laser pulse.. ""I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
