A novel poly(ε-caprolactone)/calcuim sulfate sysyem was prepared and characterized in order to enchance calcuim sulfate (gypsum) performance as bone graft substitute overcoming its brittleness and fast resorption rate. A poly (ε-caprolactone) (PCL) photo-crosslinkable derivate (PCLf) was synthesized by reaction of a low molecular weight PCL diol with methacryloyl chloride and confirmed by FT-IR and lH NMR analyses. An injectable and easy mouldable mixture of PCLf and calcium sulfate hemi-hydrate (PCLf/CHS) was obtained. Thermal analyses and solvent extraction proved the occurance of PCLf photo-crosslinking, even in the presence of CHS, in a time suitable for clinical applications. Swelling studies demonstrated that the encapsulation of the inorganic filler increase network hydrophilicity making it more permeable to water. Scanning electron microscopy, performed on crosslinked PCLf/CHS and on the feasibility to obtain, in situ, gypsum entrapped into a degradable polymeric network. in vitro cytotoxicity test, performed according to ISO 10993-5, proved that the developed system was not cytotoxic supporting its potential use in tissue engineering as a new, injectable, photocurable bone graft materials. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.
A novel poly(epsilon-caprolactone)/calcium sulfate 1 system was prepared and characterized in order to enhance calcium sulfate (gypsum) performance as bone graft substitute overcoming its brittleness and fast resorption rate. A poly(epsilon-caprolactone) (PCL) photo-crosslinkable derivative (PCLf) was synthesized by reaction of a low molecular weight PCL diol with methacryloyl chloride and confirmed by Fr-IR and H-1 NMR analyses. An injectable and easy mouldable mixture of PCLf-and calcium sulfate hemi-hydrate (PCLf/CHS) was obtained. Thermal analyses and solvent extraction proved the occurrence of PCLf-photo-crosslinking, even in the presence of CHS, in a time suitable for clinical applications. Swelling studies demonstrated that the encapsulation of the inorganic filler increases network hydrophilicity making it more permeable to water. Scanning electron microscopy, performed on crosslinked PCLf/CHS and on the same material after incubation in a PBS solution, showed the feasibility to obtain, in situ, gypsum entrapped into a degradable polymeric network. In vitro cytotoxicity tests, performed according to ISO 10993-5, proved that the developed i system was not cytotoxic supporting its potential use in tissue engineering as a new, injectable, photocurable bone graft material.
A novel injectable poly(ε-caprolactone)/calcium sulfate system for bone regeneration: Synthesis and characterization
LA GATTA, Annalisa;DE ROSA, Alfredo;DE ROSA, Mario;SCHIRALDI, Chiara
2005
Abstract
A novel poly(epsilon-caprolactone)/calcium sulfate 1 system was prepared and characterized in order to enhance calcium sulfate (gypsum) performance as bone graft substitute overcoming its brittleness and fast resorption rate. A poly(epsilon-caprolactone) (PCL) photo-crosslinkable derivative (PCLf) was synthesized by reaction of a low molecular weight PCL diol with methacryloyl chloride and confirmed by Fr-IR and H-1 NMR analyses. An injectable and easy mouldable mixture of PCLf-and calcium sulfate hemi-hydrate (PCLf/CHS) was obtained. Thermal analyses and solvent extraction proved the occurrence of PCLf-photo-crosslinking, even in the presence of CHS, in a time suitable for clinical applications. Swelling studies demonstrated that the encapsulation of the inorganic filler increases network hydrophilicity making it more permeable to water. Scanning electron microscopy, performed on crosslinked PCLf/CHS and on the same material after incubation in a PBS solution, showed the feasibility to obtain, in situ, gypsum entrapped into a degradable polymeric network. In vitro cytotoxicity tests, performed according to ISO 10993-5, proved that the developed i system was not cytotoxic supporting its potential use in tissue engineering as a new, injectable, photocurable bone graft material.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
