The human gene for plasminogen activator inhibitor type-1 (PAI-1) has been isolated and its promoter region characterized. PAI-1 regulation by glucocortiooids, transforming growth factor-β (TGF-β) and the phorbol ester PMA is shown to be exerted at the promoter level. A fragment spanning 805 nuclectides of the 5′ flanking and 72 of the 5′ untraslated region contain information enough to promote transcription and to respond to glucocorticoids when fused to a reporter gene and transfected into human fibrosarccma cells. A moderately repectitive DNA sequence, containing a TATA box, a GRE consensus, a Z-DNA forming sequence and two imperfect direct repeats at the extremities, is present a few nucleotides 5′ of the human PAI-1 gene transcription start site, raising the possiblity that this gene could have been activated by DNA insertion during evolution. © 1988 IRL Press Limited.

The regulatory region of the human plasminogen activator inhibitor type-1 (PAI-1) gene

RICCIO, Andrea;
1988

Abstract

The human gene for plasminogen activator inhibitor type-1 (PAI-1) has been isolated and its promoter region characterized. PAI-1 regulation by glucocortiooids, transforming growth factor-β (TGF-β) and the phorbol ester PMA is shown to be exerted at the promoter level. A fragment spanning 805 nuclectides of the 5′ flanking and 72 of the 5′ untraslated region contain information enough to promote transcription and to respond to glucocorticoids when fused to a reporter gene and transfected into human fibrosarccma cells. A moderately repectitive DNA sequence, containing a TATA box, a GRE consensus, a Z-DNA forming sequence and two imperfect direct repeats at the extremities, is present a few nucleotides 5′ of the human PAI-1 gene transcription start site, raising the possiblity that this gene could have been activated by DNA insertion during evolution. © 1988 IRL Press Limited.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11591/229548
Citazioni
  • ???jsp.display-item.citation.pmc??? 11
  • Scopus 72
  • ???jsp.display-item.citation.isi??? ND
social impact