9 TB patients, 4 without (Group I) and with (Group II) chest X-ray evidence of cavitary disease were evaluated at admission for the frequencies of TH1 and TH2 CD4 T-cells in the BAL fluid using intracellular immunofluorescence with anti INF-y (TH1) and anti-IL-4 (TH2) antibodies and flow-cytometric analysis and for the release in 24 h. culture supematants of IFNy. TH1 CD4+ cells dominated (93 ± 2%) the alveolar lymphocytes while TH2 CD4+ T-cells were absent in Group I patients; on the other hand, both TH1 (66 ± 4%) and TH2 CD4+ T-cells (28 ± 5%) were present in Group II patients. Also, BAL cells from Group I patients release high levels of IFNy (144 ±13 pg/ml) with minimal levels of IL-4 (<2 pg/ml) while BAL cells from Group II patients released lower IFNy (23 ± 5 pg/ml) with higher levels of IL-4 (75 ± 24 pg/ml). This suggest that IFNy secretion may be lowered by the presence of activate TH2 CD4+ cells in the inflammatory milieu.

CYTOFLUORIMETRIC ANALYSIS OF CYTOKINE-PRODUCING LYMPHOCYTES IN BAL FROM TB PATIENTS

MAZZARELLA, Gennaro;CALABRESE, Cecilia;
1997

Abstract

9 TB patients, 4 without (Group I) and with (Group II) chest X-ray evidence of cavitary disease were evaluated at admission for the frequencies of TH1 and TH2 CD4 T-cells in the BAL fluid using intracellular immunofluorescence with anti INF-y (TH1) and anti-IL-4 (TH2) antibodies and flow-cytometric analysis and for the release in 24 h. culture supematants of IFNy. TH1 CD4+ cells dominated (93 ± 2%) the alveolar lymphocytes while TH2 CD4+ T-cells were absent in Group I patients; on the other hand, both TH1 (66 ± 4%) and TH2 CD4+ T-cells (28 ± 5%) were present in Group II patients. Also, BAL cells from Group I patients release high levels of IFNy (144 ±13 pg/ml) with minimal levels of IL-4 (<2 pg/ml) while BAL cells from Group II patients released lower IFNy (23 ± 5 pg/ml) with higher levels of IL-4 (75 ± 24 pg/ml). This suggest that IFNy secretion may be lowered by the presence of activate TH2 CD4+ cells in the inflammatory milieu.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11591/225077
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