Objectives: Platelet-rich plasma (PRP) is at present used in surgical procedures as a topically-applicable source of autologous growth factors. Recently, PRP has been proposed in the field of periodontal regeneration techniques; however, little is known about its biological effects on periodontal cells in vitro. The aim of this study was to investigate whether PRP could affect the proliferation and the expression of osteoblast-like properties in primary cultures of human periodontal ligament cells (PDLC) in vitro. Methods: PRP was obtained by drawing healthy donor blood samples in presence of citric acid - sodium citrate - dextrose as anticoagulant, and centrifuged 7 min at 300xg to separate plasma and red blood cells. The top 30% of plasma was drawn off and discarded, while the remaining plasma was collected and used as PRP. Human primary PDLC at early passages (2-5) were exposed to 10% fresh PRP. Mitogenic activity, measured as 3H-thymidine incorporation, was evaluated at 48 and 72h, while the early osteoblast-like differentiation marker, namely alkaline phosphatase (ALP), was assessed at 3 and 6 days. Results: A strong, time-dependent, growth stimulation was observed that, after 48 and 72 h PDLC incubation with PRP, was approximately 1.5-fold and 3.5-fold higher than controls (P<0.001), respectively. Parallelly, PRP also induced a time-dependent increase of ALP specific activity, that ranged from 2.4 to 3.9-fold of untreated controls at 3 and 6 days (P<0.01), respectively. Conclusions: Our data demonstrate the significant PRP capability of inducing PDLC proliferation and differentiation, suggesting potential benefits in the field of periodontal regenerative techniques.

PRP stimulates proliferation and differentiation of human periodontal ligament cells

ANNUNZIATA, Marco;OLIVA, Adriana;GUIDA, Luigi
2004

Abstract

Objectives: Platelet-rich plasma (PRP) is at present used in surgical procedures as a topically-applicable source of autologous growth factors. Recently, PRP has been proposed in the field of periodontal regeneration techniques; however, little is known about its biological effects on periodontal cells in vitro. The aim of this study was to investigate whether PRP could affect the proliferation and the expression of osteoblast-like properties in primary cultures of human periodontal ligament cells (PDLC) in vitro. Methods: PRP was obtained by drawing healthy donor blood samples in presence of citric acid - sodium citrate - dextrose as anticoagulant, and centrifuged 7 min at 300xg to separate plasma and red blood cells. The top 30% of plasma was drawn off and discarded, while the remaining plasma was collected and used as PRP. Human primary PDLC at early passages (2-5) were exposed to 10% fresh PRP. Mitogenic activity, measured as 3H-thymidine incorporation, was evaluated at 48 and 72h, while the early osteoblast-like differentiation marker, namely alkaline phosphatase (ALP), was assessed at 3 and 6 days. Results: A strong, time-dependent, growth stimulation was observed that, after 48 and 72 h PDLC incubation with PRP, was approximately 1.5-fold and 3.5-fold higher than controls (P<0.001), respectively. Parallelly, PRP also induced a time-dependent increase of ALP specific activity, that ranged from 2.4 to 3.9-fold of untreated controls at 3 and 6 days (P<0.01), respectively. Conclusions: Our data demonstrate the significant PRP capability of inducing PDLC proliferation and differentiation, suggesting potential benefits in the field of periodontal regenerative techniques.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11591/219189
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