Objectives: The aim of this study was to evaluate the effect of differently treated titanium implant surfaces on the response of human bone marrow stromal cells (BMSCs) in terms of adhesion, proliferation and osteogenic differentiation. Methods: 15x1 mm titanium disks machined (M), pellet-blasted (P), sand-blasted (S), titanium-nitride sandblasted (TiN-S), titanium plasma sprayed (TPS) and titanium-nitride TPS (TiN-TPS) (P.H.I. s.r.l., San Vittore Olona, Milano, Italy) were topographically examined by atomic force microscopy and confocal optical profilometry. Primary cultures of BMSCs, obtained from bone marrow samples of healthy donors, were seeded on the titanium disks and cell adhesion and proliferation were evaluated. Furthermore, the osteogenic differentiation, in terms of alkaline phosphatase activity, osteocalcin synthesis and extracellular mineralization, was also analysed. Results: Implant surfaces showed different roughness (Sa) values (M ≤ P < S ≤ TiN-S << TiN-TPS < TPS) ranging from 0.3 to 6 µm. Cell adhesion was enhanced by rough surfaces with respect to machined ones, whereas the proliferation remained unmodified and the osteogenic parameters were reduced. No difference was found between S and TiN-S disks as well as between TPS and TiN-TPS in terms of any of the examined parameters. Conclusions: Our results evidenced that the surface roughing treatments seem to enhance the adhesion of BMSCs onto titanium implant surfaces, but not their differentiation toward the osteoblastic phenotype. Furthermore, the additive process of TiN-coating, usually applied to improve the physico-chemical properties of implant surfaces, did not alter their biological performance compared to the respective uncoated samples.

The response of bone marrow stromal cells to differently treated titanium surfaces

ANNUNZIATA, Marco;OLIVA, Adriana;LANZA, Alessandro;GUIDA, Luigi
2008

Abstract

Objectives: The aim of this study was to evaluate the effect of differently treated titanium implant surfaces on the response of human bone marrow stromal cells (BMSCs) in terms of adhesion, proliferation and osteogenic differentiation. Methods: 15x1 mm titanium disks machined (M), pellet-blasted (P), sand-blasted (S), titanium-nitride sandblasted (TiN-S), titanium plasma sprayed (TPS) and titanium-nitride TPS (TiN-TPS) (P.H.I. s.r.l., San Vittore Olona, Milano, Italy) were topographically examined by atomic force microscopy and confocal optical profilometry. Primary cultures of BMSCs, obtained from bone marrow samples of healthy donors, were seeded on the titanium disks and cell adhesion and proliferation were evaluated. Furthermore, the osteogenic differentiation, in terms of alkaline phosphatase activity, osteocalcin synthesis and extracellular mineralization, was also analysed. Results: Implant surfaces showed different roughness (Sa) values (M ≤ P < S ≤ TiN-S << TiN-TPS < TPS) ranging from 0.3 to 6 µm. Cell adhesion was enhanced by rough surfaces with respect to machined ones, whereas the proliferation remained unmodified and the osteogenic parameters were reduced. No difference was found between S and TiN-S disks as well as between TPS and TiN-TPS in terms of any of the examined parameters. Conclusions: Our results evidenced that the surface roughing treatments seem to enhance the adhesion of BMSCs onto titanium implant surfaces, but not their differentiation toward the osteoblastic phenotype. Furthermore, the additive process of TiN-coating, usually applied to improve the physico-chemical properties of implant surfaces, did not alter their biological performance compared to the respective uncoated samples.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11591/219160
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