Aim: The relationship between implant titanium surface and peri-implant soft tissues may play a key role for the maintaining of peri-implant health and, ultimately, for implant therapy success. The aim of this study was to to compare a novel nano-structured oxidized titanium implant surface with a conventional turned one in terms of topographic characteristics and biological features towards primary cultures of human gingival fibroblasts (HGF). Material and Methods: 10x10x1 mm turned (control) and oxidized (test) titanium samples (P.H.I. s.r.l., San Vittore Olona, Milano, Italy) were examined by scanning electron microscopy (SEM) and atomic force microscopy (AFM) and characterized by height, spatial and hybrid roughness parameters. Primary cultures of HGF were seeded on titanium samples and cell morphology, adhesion, proliferation and extracellular matrix deposition, in terms of type I collagen synthesis, were evaluated. Results: At SEM and AFM analyses, control and test surfaces appeared considerably different: turned samples were grooved, whereas oxidized surfaces showed a more complex micro- and nano-scaled texture, as evidenced by roughness parameters. Cell adhesion and proliferation parameters, as well as collagen synthesis, were greater on oxidized (p<0.05 at least) vs turned surfaces. Conclusion: Although both control and test samples were in the range of average roughness proper of smooth surfaces, they exhibited significantly different topographic properties at micro and nano-scale levels in terms of height, spatial and, mostly, of hybrid parameters. Furthermore, oxidized surfaces enhanced HGF adhesion, proliferation and extracellular matrix deposition, with possible clinical implications in terms of peri-implant

The response of human gingival fibroblasts to nanostructured oxidized titanium surfaces

ANNUNZIATA, Marco;OLIVA, Adriana;LANZA, Alessandro;GUIDA, Luigi
2012

Abstract

Aim: The relationship between implant titanium surface and peri-implant soft tissues may play a key role for the maintaining of peri-implant health and, ultimately, for implant therapy success. The aim of this study was to to compare a novel nano-structured oxidized titanium implant surface with a conventional turned one in terms of topographic characteristics and biological features towards primary cultures of human gingival fibroblasts (HGF). Material and Methods: 10x10x1 mm turned (control) and oxidized (test) titanium samples (P.H.I. s.r.l., San Vittore Olona, Milano, Italy) were examined by scanning electron microscopy (SEM) and atomic force microscopy (AFM) and characterized by height, spatial and hybrid roughness parameters. Primary cultures of HGF were seeded on titanium samples and cell morphology, adhesion, proliferation and extracellular matrix deposition, in terms of type I collagen synthesis, were evaluated. Results: At SEM and AFM analyses, control and test surfaces appeared considerably different: turned samples were grooved, whereas oxidized surfaces showed a more complex micro- and nano-scaled texture, as evidenced by roughness parameters. Cell adhesion and proliferation parameters, as well as collagen synthesis, were greater on oxidized (p<0.05 at least) vs turned surfaces. Conclusion: Although both control and test samples were in the range of average roughness proper of smooth surfaces, they exhibited significantly different topographic properties at micro and nano-scale levels in terms of height, spatial and, mostly, of hybrid parameters. Furthermore, oxidized surfaces enhanced HGF adhesion, proliferation and extracellular matrix deposition, with possible clinical implications in terms of peri-implant
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11591/218500
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