Olive oil mill wastewater (OMW) was tested for phytotoxicity on seed germination and seedling growth of radish and wheat. The most potent inhibition was observed with the reverse osmosis fraction, from this, 17 polyphenols with molecular weight less than 300 Daltons were isolated by chromatographic processes and identified on the basis of their spectroscopic features. In seed bioassays, the inhibitory activity of each polyphenol was much lower than that observed for the original fraction and the mixture of the pure 17 polyphenols present in reverse osmosis fraction had less effect on seed germination. Thus, OMW phytotoxicity could be due to a synergic action of polyphenols with other unidentified substances present in the wastewater.
Olive oil mill wastewater: Isolation of polyphenols and their phytotoxicity in vitro
FIORENTINO, Antonio;OLIVA, Adriana;
2002
Abstract
Olive oil mill wastewater (OMW) was tested for phytotoxicity on seed germination and seedling growth of radish and wheat. The most potent inhibition was observed with the reverse osmosis fraction, from this, 17 polyphenols with molecular weight less than 300 Daltons were isolated by chromatographic processes and identified on the basis of their spectroscopic features. In seed bioassays, the inhibitory activity of each polyphenol was much lower than that observed for the original fraction and the mixture of the pure 17 polyphenols present in reverse osmosis fraction had less effect on seed germination. Thus, OMW phytotoxicity could be due to a synergic action of polyphenols with other unidentified substances present in the wastewater.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
