Human lymphoblasts deficient in iduronate sulfatase or in α-N-acetylglucosaminidase acquire discrete levels of enzyme activity after co-culture with human normal skin fibroblasts. This occurs by direct cell-to-cell contact and not by uptake of secreted fibroblast enzyme. The process is dependent on time and on the number of fibroblasts used. Electron-microscopic examination of the co-culture of the two cell types reveals extensive region of intimate contact. Fibroblastic projections appear frequently in close apposition witt lymphoblast invaginations; a diffuse micropinocytotic activity is evident only in fibroblastic cells. © 1992.
Cell-to-cell contact between normal fibroblasts and lymphoblasts deficient in lysosomal enzymes
DANIELE, Aurora;
1992
Abstract
Human lymphoblasts deficient in iduronate sulfatase or in α-N-acetylglucosaminidase acquire discrete levels of enzyme activity after co-culture with human normal skin fibroblasts. This occurs by direct cell-to-cell contact and not by uptake of secreted fibroblast enzyme. The process is dependent on time and on the number of fibroblasts used. Electron-microscopic examination of the co-culture of the two cell types reveals extensive region of intimate contact. Fibroblastic projections appear frequently in close apposition witt lymphoblast invaginations; a diffuse micropinocytotic activity is evident only in fibroblastic cells. © 1992.File in questo prodotto:
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