The response to heat shock has been examined In two strains of the dimorphic pathogenic fungus Histoplasma capsulatum, which differ considerably in thermotolerance and pathogenicity. The gene for the 70 kD heat shock protein (hsp70) was isolated using a Drosophila hsp70 gene to screen a cosmid library of the DNA from the temperature‐sensitive Downs strain (low level of thermotolerance for mice). Using the cloned gene as a probe, we have measured the transcription of the endogenous hsp70 gene at 25°C and in response to temperature shift to 34°, 37° and 40°C, temperatures that trigger the mycelial to yeast phase transition in this fungus. The gene is constitutively transcribed at low levels, both in the yeast and the mycelial stages. Synthesis of hsp70 mRNA was transiently increased 1 to 3 h after the temperature shifts. By Northern analysis, peak levels of transcription were shown to occur at 34°C in the Downs strain and at 37°C in the more pathogenic G222B strain. Our results are consistent with reports in which it has been shown that heat shock gene expression is part of temperature adaptation and probably developmental processes. The low levels of transcription of the hsp70 gene in the Downs strain at 37°C correlate with its greater temperature sensitivity and low level of virulence. Copyright © 1987, Wiley Blackwell. All rights reserved

Heat shock 70 gene is differentially expressed in Histoplasma capsulatum strains with different levels of thermotolerance and pathogenicity

SACCO, Margherita;
1987

Abstract

The response to heat shock has been examined In two strains of the dimorphic pathogenic fungus Histoplasma capsulatum, which differ considerably in thermotolerance and pathogenicity. The gene for the 70 kD heat shock protein (hsp70) was isolated using a Drosophila hsp70 gene to screen a cosmid library of the DNA from the temperature‐sensitive Downs strain (low level of thermotolerance for mice). Using the cloned gene as a probe, we have measured the transcription of the endogenous hsp70 gene at 25°C and in response to temperature shift to 34°, 37° and 40°C, temperatures that trigger the mycelial to yeast phase transition in this fungus. The gene is constitutively transcribed at low levels, both in the yeast and the mycelial stages. Synthesis of hsp70 mRNA was transiently increased 1 to 3 h after the temperature shifts. By Northern analysis, peak levels of transcription were shown to occur at 34°C in the Downs strain and at 37°C in the more pathogenic G222B strain. Our results are consistent with reports in which it has been shown that heat shock gene expression is part of temperature adaptation and probably developmental processes. The low levels of transcription of the hsp70 gene in the Downs strain at 37°C correlate with its greater temperature sensitivity and low level of virulence. Copyright © 1987, Wiley Blackwell. All rights reserved
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11591/203376
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