An improved protocol for the synthesis of oligonucleotides containing a 3′-3′ phosphodiester linkage, for use in an alternate strand triple-helix formation approach, is reported. Three 16-mers and one 24-mer have been efficiently synthesized and the corresponding triplexes have been analyzed by thermal denaturation experiments and CD spectroscopy.
A facile solid-phase synthesis of oligonucleotides containing a 3′-3′ phosphodiester bond for alternate strand triple-helix formation
MESSERE, Anna;
1998
Abstract
An improved protocol for the synthesis of oligonucleotides containing a 3′-3′ phosphodiester linkage, for use in an alternate strand triple-helix formation approach, is reported. Three 16-mers and one 24-mer have been efficiently synthesized and the corresponding triplexes have been analyzed by thermal denaturation experiments and CD spectroscopy.File in questo prodotto:
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