We have identified and characterized a highly repetitive family, called R.e./Tc1 in the genome of the green water frog Rana esculenta. This family consists of tandemly repeated sequences, localized at the centromeric regions of chromosomes as shown by Southern blot and 'in situ' hybridization. The repeat unit contains a residue of a Tc1-like transposon by Haematobia irritans fly, bordered by two short direct repeats of 9 bp. Tc1 remnant lays near a sequence identical to Homo sapiens Werner syndrome gene stretch. These sequence data suggest that R.e./Tc1 element was probably originated from a transposition event and a duplication via DNA mechanism of the R.e./Tc1 unit that could give rise to the observed tandem array. © 2000 Elsevier Science B.V.
A novel repeated sequence DNA originated from a Tc1-like transposon in water green frog Rana esculenta
DE FELICE, Bruna;
2000
Abstract
We have identified and characterized a highly repetitive family, called R.e./Tc1 in the genome of the green water frog Rana esculenta. This family consists of tandemly repeated sequences, localized at the centromeric regions of chromosomes as shown by Southern blot and 'in situ' hybridization. The repeat unit contains a residue of a Tc1-like transposon by Haematobia irritans fly, bordered by two short direct repeats of 9 bp. Tc1 remnant lays near a sequence identical to Homo sapiens Werner syndrome gene stretch. These sequence data suggest that R.e./Tc1 element was probably originated from a transposition event and a duplication via DNA mechanism of the R.e./Tc1 unit that could give rise to the observed tandem array. © 2000 Elsevier Science B.V.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
