The role of androgens in the cyclic secretory activity of the Rana esculenta Harderian gland (HG) was studied. Total RNA showed a dramatic increase in October and May when the nuclear androgen receptors peak. During the resumption of the secretory activity a gradual increase of poly(A)+-RNA was detected; during the enhancement phase (May) a peak of the poly(A)+-RNA fraction was found. In in vitro experiments testosterone increased the incorporation of [3H]uridine into the poly(A)+-RNA fraction and also that of [35S]methionine into a newly synthesized protein fraction (100 kDa). The latter effect is prevented by the exposure of the cells to the antiandrogen, cyproterone acetate (CPA). These findings reveal that, besides hamsters, the HG is a target for androgens in the frog. © 1992.
TESTOSTERONE INDUCTION OF POLY(A)+-RNA SYNTHESIS AND [S-35] METHIONINE INCORPORATION INTO PROTEINS OF RANA-ESCULENTA HARDERIAN-GLAND
VARRIALE B;CHIEFFI-BACCARI Gabriella;D'ISTRIA M;DI MATTEO L;MINUCCI S;SERINO I;
1992
Abstract
The role of androgens in the cyclic secretory activity of the Rana esculenta Harderian gland (HG) was studied. Total RNA showed a dramatic increase in October and May when the nuclear androgen receptors peak. During the resumption of the secretory activity a gradual increase of poly(A)+-RNA was detected; during the enhancement phase (May) a peak of the poly(A)+-RNA fraction was found. In in vitro experiments testosterone increased the incorporation of [3H]uridine into the poly(A)+-RNA fraction and also that of [35S]methionine into a newly synthesized protein fraction (100 kDa). The latter effect is prevented by the exposure of the cells to the antiandrogen, cyproterone acetate (CPA). These findings reveal that, besides hamsters, the HG is a target for androgens in the frog. © 1992.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
