Estrogen receptors alpha (ERα) and beta (ERβ) play distinct biological roles in onset and progression of hormone-responsive breast cancer (BC), with ERβ exerting a modulatory activity on ERα-mediated estrogen signalling and stimulation of cell proliferation by mechanisms still not fully understood. We stably expressed human ERβ fused to a TAP-tag in estrogen-responsive MCF-7 cells and applied Tandem Affinity Purification (TAP) and nanoLC-MS/MS to identify the ERβ interactome of this cell type. Functional annotation by bioinformatics analyses of the 303 proteins that co-purify with ERβ from nuclear extracts identify several new molecular partners of this receptor subtype that represents nodal points of a large protein network controlling multiple processes and functions in BC cells.
A large set of estrogen receptor β-interacting proteins identified by tandem affinity purification in hormone-responsive human breast cancer cell nuclei.
NOLA, Ernesto;
2011
Abstract
Estrogen receptors alpha (ERα) and beta (ERβ) play distinct biological roles in onset and progression of hormone-responsive breast cancer (BC), with ERβ exerting a modulatory activity on ERα-mediated estrogen signalling and stimulation of cell proliferation by mechanisms still not fully understood. We stably expressed human ERβ fused to a TAP-tag in estrogen-responsive MCF-7 cells and applied Tandem Affinity Purification (TAP) and nanoLC-MS/MS to identify the ERβ interactome of this cell type. Functional annotation by bioinformatics analyses of the 303 proteins that co-purify with ERβ from nuclear extracts identify several new molecular partners of this receptor subtype that represents nodal points of a large protein network controlling multiple processes and functions in BC cells.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
