Using an anti-Fos family member antibody, we have previously described in Rana esculenta testis the presence of a nuclear, 43 kDa protein that we hypothesized to be Fra-1. With the assistance of an antibody against Fra-1 that does not crossreact with other Fos family members, here we report data on Fra-1 expression, localization, and putative activity in Rana esculenta testis during its annual reproductive cycle.Western blot analysis confirms that the nuclear, 43 kDa protein is Fra-1. Immunocytochemistry validates the Western blot results and shows cytoplasmic and nuclear immunostaining of Fra-1 in peritubular myoid cells, efferent ducts, and blood vessels. We report for the first time in a vertebrate, experimental evidence showing that the expression of Fra-1 is related to peritubular myoid cells during sperm transport from the tubular compartment to efferent ducts.
Fra-1 activity in the frog, Rana esculenta, testis: a new potential role in sperm transport.
COBELLIS, Gilda;PIERANTONI, Riccardo;FASANO, Silvia
2005
Abstract
Using an anti-Fos family member antibody, we have previously described in Rana esculenta testis the presence of a nuclear, 43 kDa protein that we hypothesized to be Fra-1. With the assistance of an antibody against Fra-1 that does not crossreact with other Fos family members, here we report data on Fra-1 expression, localization, and putative activity in Rana esculenta testis during its annual reproductive cycle.Western blot analysis confirms that the nuclear, 43 kDa protein is Fra-1. Immunocytochemistry validates the Western blot results and shows cytoplasmic and nuclear immunostaining of Fra-1 in peritubular myoid cells, efferent ducts, and blood vessels. We report for the first time in a vertebrate, experimental evidence showing that the expression of Fra-1 is related to peritubular myoid cells during sperm transport from the tubular compartment to efferent ducts.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
