We investigated the role of inducible nitric oxide synthase (iNOS) on ischemic myocardial damage and angiogenic process in genetically deficient iNOS (iNOS-/-) mice and wild-type littermates (iNOS-/-), with and without streptozotocin-induced (70 mg/kg intra-venously)diabetes. After ischemia (25 min) and reperfusion (120 min), both iNOS-/- and iNOS-/- diabetic mice (blood glucose 22 mmol/l) had myocardial infarct size greater than their respective nondiabetic littermates (P < 0.01). Myocardial infarct size (P < 0.05), apoptotic index (P < 0.005), and tissue levels of tumor necrosis factor (P < 0.01), interleukin-6 (P < 0.01), and interleukin-18 (P < 0.01) were higher in nondiabetic iNOS-/- mice compared with nondiabetic iNOS-/- mice. As compared with diabetic iNOS-/- mice, diabetic iNOS-/- mice showed a greater infarct size (P < 0.01) associated with the highest tissue levels of nitrotyrosine and proinflammatory cytokines, as well as apoptosis. The beneficial role of iNOS in modulating defensive responses against ischemia/reperfusion injury seems to be abolished in diabetic mice.
Absence of inducible nitric oxide synthase reduces myocardial damage during ischemia reperfusion in streptozotocin-induced hyperglycemic mice
MARFELLA, Raffaele;DI FILIPPO, Clara;ESPOSITO, Katherine;PIEGARI, Elena;BERRINO, Liberato;ROSSI, Francesco;GIUGLIANO, Dario;D'AMICO, Michele
2004
Abstract
We investigated the role of inducible nitric oxide synthase (iNOS) on ischemic myocardial damage and angiogenic process in genetically deficient iNOS (iNOS-/-) mice and wild-type littermates (iNOS-/-), with and without streptozotocin-induced (70 mg/kg intra-venously)diabetes. After ischemia (25 min) and reperfusion (120 min), both iNOS-/- and iNOS-/- diabetic mice (blood glucose 22 mmol/l) had myocardial infarct size greater than their respective nondiabetic littermates (P < 0.01). Myocardial infarct size (P < 0.05), apoptotic index (P < 0.005), and tissue levels of tumor necrosis factor (P < 0.01), interleukin-6 (P < 0.01), and interleukin-18 (P < 0.01) were higher in nondiabetic iNOS-/- mice compared with nondiabetic iNOS-/- mice. As compared with diabetic iNOS-/- mice, diabetic iNOS-/- mice showed a greater infarct size (P < 0.01) associated with the highest tissue levels of nitrotyrosine and proinflammatory cytokines, as well as apoptosis. The beneficial role of iNOS in modulating defensive responses against ischemia/reperfusion injury seems to be abolished in diabetic mice.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
