Determination of miRNAs from cancer stem cells using a low density array platform

A microarray approach has been extensively used for global gene expression profiles in many biological research fields such as understanding of pathological mechanism in malignancies and defining of molecular biomarkers to monitor disease status. The most attractive advantage of microarray technology is its application to simultaneous analysis of miRNA expression pattern with a large amount of assessments. In this chapter, we provide a facile and universal protocol for divergent miRNA expression profiles in prostate cancer stem cells with a low density array-based microarray analysis.