We have recently analyzed in mouse embryo NIH3T3 fibroblasts and human fibrosarcoma HT1080 cells the molecular basis and the biological role of AR interaction with the full-length filamin A (FLNa), an actin-cross-linking protein. Here, we describe a procedure revealing the AR/FLNa complex in stromal cells. Upon physiological (10 nM) androgen stimulation of quiescent NIH3T3 cells, FLNa co-immunoprecipitates with AR and co-localizes with the receptor at intermediate actin filaments. The AR/FLNa complex specifically regulates AR extranuclear functions leading to Rac1 activation and consequent cell motility. This complex adds a new and unexpected piece to the growing evidence of the role of signalling effectors, scaffolds, and cytoskeletal proteins in the rapid androgen action and in progression of hormone-dependent cancers.

Analysis of the Androgen Receptor/Filamin A Complex in Stromal Cells

Giovannelli P;Di Donato M;CASTORIA, Gabriella
2014

Abstract

We have recently analyzed in mouse embryo NIH3T3 fibroblasts and human fibrosarcoma HT1080 cells the molecular basis and the biological role of AR interaction with the full-length filamin A (FLNa), an actin-cross-linking protein. Here, we describe a procedure revealing the AR/FLNa complex in stromal cells. Upon physiological (10 nM) androgen stimulation of quiescent NIH3T3 cells, FLNa co-immunoprecipitates with AR and co-localizes with the receptor at intermediate actin filaments. The AR/FLNa complex specifically regulates AR extranuclear functions leading to Rac1 activation and consequent cell motility. This complex adds a new and unexpected piece to the growing evidence of the role of signalling effectors, scaffolds, and cytoskeletal proteins in the rapid androgen action and in progression of hormone-dependent cancers.
Giovannelli, P; Di Donato, M; Auricchio, F; Castoria, Gabriella
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11591/225842
Citazioni
  • ???jsp.display-item.citation.pmc??? 3
  • Scopus 5
  • ???jsp.display-item.citation.isi??? 5
social impact