In this chapter, we have indicated promising surface markers, such as CD133, CD117 and Stro-1, for the identification of candidate osteosarcoma CSCs and at the same time of potential therapeutic targets. Refinements in flow cytometric techniques have allowed for the identification of SPs cells and ALDHbright cells which show stem cell-like properties, including self-renewal, tumor initiation, serial propagation and resistance to cytotoxic chemotherapy agents. Further technical refinements and the use of novel techniques, such as genetic lineage tracking, may allow the identification and isolation of a purer population of CSCs from osteosarcomas. Improved understanding of the cellular origins of osteosarcomas, and further refinements in the molecular characterization of these tumors, will be essential in the discrimination of putative CSCs.
Stemness markers of osteosarcoma
TIRINO, Virginia;PAINO, Francesca;La Noce M;PAPACCIO, Gianpaolo;DESIDERIO, Vincenzo
2014
Abstract
In this chapter, we have indicated promising surface markers, such as CD133, CD117 and Stro-1, for the identification of candidate osteosarcoma CSCs and at the same time of potential therapeutic targets. Refinements in flow cytometric techniques have allowed for the identification of SPs cells and ALDHbright cells which show stem cell-like properties, including self-renewal, tumor initiation, serial propagation and resistance to cytotoxic chemotherapy agents. Further technical refinements and the use of novel techniques, such as genetic lineage tracking, may allow the identification and isolation of a purer population of CSCs from osteosarcomas. Improved understanding of the cellular origins of osteosarcomas, and further refinements in the molecular characterization of these tumors, will be essential in the discrimination of putative CSCs.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
