In this invention is described a method that foresees the iso-lation of a new subpopulation of stem cells derived form dental pulp, whose differentiation is osteoblasts lead to the subsequent production and employment of a bone tissue, called LAB (Living Autologous Bone). Specifically, the invention describes: 1) the isolation of stem cells from the pulp of deciduous and permanent teeth and of dental genns, obtained from human subjects; 2) the growth of these cells in vitro, under specific conditions that allow the isolation of a cellular sub-population, which, after differentiation in osteo¬blasts, is able to produce in vitro an extracellular matrix, identical to that detectable in bone tissue; 3) the use of this selected and differentiated celi population in arder to produce autologous bone tissue in vitro, containing vital osteoblasts; 4) the preservation of the LAB under conditions which guar¬antee cellular vitality; 5) the use of the LAB in donor patients to reconstruct bone tissue, as required in the daily practice in dentistry, maxillo-facial surgery and orthopedics.
STEM CELLS OBTAINED FROM PULP OF DECIDUOUS OR PERMANENT TEETH AND OF DENTAL GERM,ABLE TO PRODUCE HUMAN BONE TISSUE
DE ROSA, Alfredo;LAINO, Gregorio;PAPACCIO, Gianpaolo
2004
Abstract
In this invention is described a method that foresees the iso-lation of a new subpopulation of stem cells derived form dental pulp, whose differentiation is osteoblasts lead to the subsequent production and employment of a bone tissue, called LAB (Living Autologous Bone). Specifically, the invention describes: 1) the isolation of stem cells from the pulp of deciduous and permanent teeth and of dental genns, obtained from human subjects; 2) the growth of these cells in vitro, under specific conditions that allow the isolation of a cellular sub-population, which, after differentiation in osteo¬blasts, is able to produce in vitro an extracellular matrix, identical to that detectable in bone tissue; 3) the use of this selected and differentiated celi population in arder to produce autologous bone tissue in vitro, containing vital osteoblasts; 4) the preservation of the LAB under conditions which guar¬antee cellular vitality; 5) the use of the LAB in donor patients to reconstruct bone tissue, as required in the daily practice in dentistry, maxillo-facial surgery and orthopedics.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
