Plasmonic immunosensors based on POF D-shaped for detection of ochratoxin A

The high toxicity and occurrence of ochratoxin A (OTA) in grains and foods has been a growing concern due to the impacts on health and the economy in many countries. In this sense, simplified devices with high sensitivity and specificity for local monitoring are enthusiastically pursued. In this work, we report for the first time the detection of ochratoxin As using a POF D-Shaped immunosensor. The biosensor was built with the surface of the POF D-Shaped covered by a 60 nm layer of gold to enable the SPR phenomenon. An optical immunosensor based on SPR phenomena, was successfully developed for the ultra-low detection of OTA. The calibration curve provided a linear range from x ppb to x ppb. Furthermore, the developed biosensor represents a promising analytical device for coffee quality analyses, as it is portable, simple, and suitable for onsite detection of target analytes without microfluidic systems.