In this work, the advantages of 3D printing were combined with the use of UV-cured optical adhesives in order to obtain plasmonic biosensors of the desired shape and adaptable to different experimental setups. Using 3D printers, it is possible to design and manufacture tailored holders, which, combined with UV-cured optical adhesives covered by multilayer, enable the production of plasmonic chips with high sensitivity. Following a functionalization process, a specific receptor layer over the plasmonic nanofilm can be realized to achieve the bio/chemical sensor for detecting substances of interest, such as biomarkers, viruses, bacteria, and pollutants. As a proof-of-concept, a plasmonic biosensor for the specific detection of IGG protein was realized and tested in this work in order to show the detection capability at the femtomolar level. The experimental results have shown a detection limit of approximately 1 fM. The proposed approach could be used to develop disposable biochips for small-size, low-cost, easy-to-use, and connected to the Internet Point-Of-Care Tests (POCTs).
Towards Point-of-Care Tests via Polymer-Based Plasmonic Biosensors for Femtomolar-Level Detection of Proteins
Marzano C.;Arcadio F.;Zeni L.;Tavoletta I.;Renzullo L. P.;Cennamo N.
2024
Abstract
In this work, the advantages of 3D printing were combined with the use of UV-cured optical adhesives in order to obtain plasmonic biosensors of the desired shape and adaptable to different experimental setups. Using 3D printers, it is possible to design and manufacture tailored holders, which, combined with UV-cured optical adhesives covered by multilayer, enable the production of plasmonic chips with high sensitivity. Following a functionalization process, a specific receptor layer over the plasmonic nanofilm can be realized to achieve the bio/chemical sensor for detecting substances of interest, such as biomarkers, viruses, bacteria, and pollutants. As a proof-of-concept, a plasmonic biosensor for the specific detection of IGG protein was realized and tested in this work in order to show the detection capability at the femtomolar level. The experimental results have shown a detection limit of approximately 1 fM. The proposed approach could be used to develop disposable biochips for small-size, low-cost, easy-to-use, and connected to the Internet Point-Of-Care Tests (POCTs).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
