The aim of the present study was to synthetize new phenol-based materials through the sol gel approach. 5-O-ceaffeoyl quinie acid, a natural antioxidant better known as chlorogenic acid (CGA), was incorporated within silica matrix obtaining orange colored hybrid materials, characterized by Fourier Transform InfraRed (FTIR) and Ultra Violet-Visible (UV-Vis) spectroscopic techniques. In vitro soaking tests in simulated body fluid (SI3F) was performed to evaluate the new materials' bioactivity and the formation of a hydroxyapatite layer was observed on the hybrid surface by SEM/EDX analysis. Moreover, CGA-based materials were biocompatible to murine and mammalian cells as assessed by tetrazolium dye (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)-based microtitration assay (MIT assay) and exerted antioxidant capability by scavenging DPPH and ARTS(+) radicals.
Sol-Gel Processing for new silica based materials functionalized with chlorogenic acid
Michelina Catauro
;Severina Pacifico
2018
Abstract
The aim of the present study was to synthetize new phenol-based materials through the sol gel approach. 5-O-ceaffeoyl quinie acid, a natural antioxidant better known as chlorogenic acid (CGA), was incorporated within silica matrix obtaining orange colored hybrid materials, characterized by Fourier Transform InfraRed (FTIR) and Ultra Violet-Visible (UV-Vis) spectroscopic techniques. In vitro soaking tests in simulated body fluid (SI3F) was performed to evaluate the new materials' bioactivity and the formation of a hydroxyapatite layer was observed on the hybrid surface by SEM/EDX analysis. Moreover, CGA-based materials were biocompatible to murine and mammalian cells as assessed by tetrazolium dye (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)-based microtitration assay (MIT assay) and exerted antioxidant capability by scavenging DPPH and ARTS(+) radicals.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
