2NMR-based metabonomics of exhaled breath condensate (EBC) is able to significantly recognize healthy subjects from patients affected by COPD, identifying specific profiles linked to cellular oxidative pattern. Curcumin has been very recently recognized as an anti-inflammatory and anti-oxidant agent in the biochemical cellular balance, through the specific activation of Nrf2. Nrf2 has been shown to play a pivotal role in protecting cells from oxidative stress and inflammation, by the induction of cellular stress response genes, such as HO-1 and phase II enzymes. Several studies have also demonstrated an essential role of Nrf2 as a key element in modulation of macrophage activation in lung in response to cigarette smoke and to LPS, and its downregulation has been strongly associated to the etiology of COPD. In this preliminary open label study we aimed to evaluate the effect of daily oral administration of a preparation containing high dosage of a curcumin-based herbal preparation on EBC in a cohort of COPD patients by using NMR spectroscopy. 10 COPD (63.3 ± 7.9) patients were evaluated at baseline (time 0) and after 12 weeks (time 1) of oral administration of curcumin once a day, collecting during each visit EBC samples, clinical (breath, cough, sputum and night awakening, BSCN, score) data and spirometry. EBC collected from each patient at time 0 and after treatment were different in NMR spectra profiles. Notably, the profiles from treated patients displayed many new resonances appearing in the spectra. Moreover, patients examined two months after the administration period demonstrated EBC profiles similar to those at baseline. Among clinical parameters, BSCN score was 4.9 ± 0.9 at baseline and 7.5 ± 1.0 at time 1, while FEV1 alues resulted slightly but not significantly increased at the end of treatment. NMR metabonomic is a sensitive method to explore the nutraceutical effects of phytochemicals on EBC in patients affected by COPD. A corollary of our study is that curcumin, probably via the activation of Nrf2 nuclear translocation, efficiently improve endogenous antioxidant defenses in lungs, and should be evaluated as a new therapeutic approach for treatment and prevention of pulmonary diseases that correlate with oxidative damage, such as COPD.

NMR-based metabonomic assessment to investigate the effects of curcumin in COPD: a pilot study

BIANCO, Andrea
2012

Abstract

2NMR-based metabonomics of exhaled breath condensate (EBC) is able to significantly recognize healthy subjects from patients affected by COPD, identifying specific profiles linked to cellular oxidative pattern. Curcumin has been very recently recognized as an anti-inflammatory and anti-oxidant agent in the biochemical cellular balance, through the specific activation of Nrf2. Nrf2 has been shown to play a pivotal role in protecting cells from oxidative stress and inflammation, by the induction of cellular stress response genes, such as HO-1 and phase II enzymes. Several studies have also demonstrated an essential role of Nrf2 as a key element in modulation of macrophage activation in lung in response to cigarette smoke and to LPS, and its downregulation has been strongly associated to the etiology of COPD. In this preliminary open label study we aimed to evaluate the effect of daily oral administration of a preparation containing high dosage of a curcumin-based herbal preparation on EBC in a cohort of COPD patients by using NMR spectroscopy. 10 COPD (63.3 ± 7.9) patients were evaluated at baseline (time 0) and after 12 weeks (time 1) of oral administration of curcumin once a day, collecting during each visit EBC samples, clinical (breath, cough, sputum and night awakening, BSCN, score) data and spirometry. EBC collected from each patient at time 0 and after treatment were different in NMR spectra profiles. Notably, the profiles from treated patients displayed many new resonances appearing in the spectra. Moreover, patients examined two months after the administration period demonstrated EBC profiles similar to those at baseline. Among clinical parameters, BSCN score was 4.9 ± 0.9 at baseline and 7.5 ± 1.0 at time 1, while FEV1 alues resulted slightly but not significantly increased at the end of treatment. NMR metabonomic is a sensitive method to explore the nutraceutical effects of phytochemicals on EBC in patients affected by COPD. A corollary of our study is that curcumin, probably via the activation of Nrf2 nuclear translocation, efficiently improve endogenous antioxidant defenses in lungs, and should be evaluated as a new therapeutic approach for treatment and prevention of pulmonary diseases that correlate with oxidative damage, such as COPD.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11591/329270
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