Synthesis and characterization of new 3 '-3 ' linked oligodeoxyribonucleotides for alternate strand triple helix formation

Protected forms of 1,2,3-propanetriol and cis, cis-1,3,5- cyclohexanetriol were incorporated onto solid supports which were exploited in the solid phase synthesis of 3'-3' linked oligodeoxyribonucleotides (ODNs), involving only nucleoside 3'-phosphoramidites as building blocks. UV thermal denaturation analysis showed the ability of ODNs with this inversion of polarity motif to cooperatively hybridize with duplexes of the type 5'- (Pu)(m)(Py)(n)-3' in an alternate strand recognition approach.