To control and prevent the oxidative stress plants synthesize many antioxidant metabolites among which tocopherols are the most important lipophilic ones, playing a key role in preserving membrane structures. They are synthesized exclusively by photosynthetic organisms and are acquired by animals through the diet. Vegetables, during storage, suffer qualitative and quantitative changes that can affect tocopherols as well as other nutritional, nutraceutic and organoleptic characteristics. In this work tocopherols have been studied in broccoli from Brassica rapa cv. sylvestris kept in different conditions (4°C, 9°C, 20°C) and stored for different time intervals from the harvest. Sample materials (in triplicate) were extracted in methanol and used to determine alpha and gamma tocopherols. Such compounds were separated by reverse phase HPLC, detected fluorimetrically and quantified by comparison with pure standard compounds. The results show that the storage at 4°C better preserves the tocopherol contents. Other results were also discussed. The work was financially supported by "Seconda Università degli Studi di Napoli" and "MIUR"•(PRIN2006077008_005;2008S9T3KK_003).

Effect of storage temperature on vitamin E content (tocopherols) in broccoli Brassica rapa L. subsp. sylvestris

CARILLO, Petronia;FUGGI, Amodio
2010

Abstract

To control and prevent the oxidative stress plants synthesize many antioxidant metabolites among which tocopherols are the most important lipophilic ones, playing a key role in preserving membrane structures. They are synthesized exclusively by photosynthetic organisms and are acquired by animals through the diet. Vegetables, during storage, suffer qualitative and quantitative changes that can affect tocopherols as well as other nutritional, nutraceutic and organoleptic characteristics. In this work tocopherols have been studied in broccoli from Brassica rapa cv. sylvestris kept in different conditions (4°C, 9°C, 20°C) and stored for different time intervals from the harvest. Sample materials (in triplicate) were extracted in methanol and used to determine alpha and gamma tocopherols. Such compounds were separated by reverse phase HPLC, detected fluorimetrically and quantified by comparison with pure standard compounds. The results show that the storage at 4°C better preserves the tocopherol contents. Other results were also discussed. The work was financially supported by "Seconda Università degli Studi di Napoli" and "MIUR"•(PRIN2006077008_005;2008S9T3KK_003).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11591/215236
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