ODNs containing a 3′-3′ phosphodiester junction have been conveniently synthesized through a solid phase procedure involving only 3′-phosphoramidite nucleosides, starting from a modified support linking the first nucleotide through the base. Thermal denaturation studies showed that 16-mers with this inversion of polarity cooperatively bound to 5′-(purine)m(pyrimidine)n-3′-type duplexes by specific recognition of the oligopurine domains alternately on the two DNA strands. The nature of the 3′-3′ junction and nearest neighbors affected the stability of the resulting triplexes.
Synthesis and triple helix formation by alternate strand recognition of oligonucleotides containing 3 '-3 ' phosphodiester bonds
MESSERE, Anna;
1997
Abstract
ODNs containing a 3′-3′ phosphodiester junction have been conveniently synthesized through a solid phase procedure involving only 3′-phosphoramidite nucleosides, starting from a modified support linking the first nucleotide through the base. Thermal denaturation studies showed that 16-mers with this inversion of polarity cooperatively bound to 5′-(purine)m(pyrimidine)n-3′-type duplexes by specific recognition of the oligopurine domains alternately on the two DNA strands. The nature of the 3′-3′ junction and nearest neighbors affected the stability of the resulting triplexes.File in questo prodotto:
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